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However, the activities of its UT and oxidized forms were 1.3-fold lower than that of the wild-type

For example, biochemical assays for desensitization, such as inhibition of adenylyl cyclase that requires more than 5 minutes of sustained opioid exposure (most assays take 1020 minutes, e.g., Law et al., 2000, or longer, e.g., Koch et al., 2005), measure the combined effects of rapid desensitization at the cell surface plus endocytosis or recovery from desensitization